Properties：

Overview：

Description：
Uraciline-DNA glycosylase (UDG) excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the glycoskelet. This cleavage produces base-sensitive pyrimidine sites that are blocked by DNA polymerase from replicating or prevented from becoming hybridization sites. Only double- and single-stranded dU-containing DNA can be recognized and degraded by uracil-DNA glycosylase, while RNA and normal dT-containing DNA do not. Uracil-DNA glycosylase from cold-water fish is thermally unstable. Complete irreversible inactivation occurs after 10 min of incubation at 50 °C.

Definition of Active Unit
● The amount of enzyme required to degrade 1 μg of uracil-containing dsDNA in 30 min at 25°C is defined as 1 active unit (U).

Features and Benefits
● Prevents contamination of PCR residues.
● Improved efficiency of site-directed mutation generation processes.
● Labeling oligonucleotide probes.
● Faster inactivation due to lower thermal stability than corresponding enzymes from E. coli.
● Does not degrade dU-PCR products by incubation at +2 to +8°C for at least a few hours

Applications
● DNA repair and mutation detection.
● Improve the cloning efficiency of PCR products.
● Improve the efficiency of fixed-point mutagenesis.
● Study of protein-DNA interactions.
● Eliminate aerosol contamination during PCR, qPCR and RT-qPCR amplification