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Lateral Flow
Lateral Flow has become the most popular technique used for POCT application for in vitro diagnostic. This technique emerged in the early 80s of the 20th century as a simple and rapid immunological detection method based on the combination of immunotechnology and chromatographic chromatography.

The principle of lateral flow immunoassay technology is to immobilize specific antigens or antibodies on nitrocellulose membranes (NC membranes) in bands, and adsorb another labeled antigen or antibody from sample pad, which has reacted with samples sufficiently.

First, the sample is added to the sample pad at one side of the test strip, which move through sample pad and dissolves and reacts with labeled antigen or antibody through capillarity Second, The antigen-antibody complex move forward and are captured the immobilized specific antibodies on NC membranes specifically, which can be detected and showed red or any other special visible color. And negative result is always showed blank.

Therefore, lateral flow has been developed rapidly in recent years which has been widely used in food safety, disease diagnosis, drug detection, environmental monitoring, etc. Compared to other techniques such as enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase chain reaction (QPCR), the result by lateral flow has showed strong advantages:

1. One-step test, no redundant cleaning process
2. Faster, lower-cost, and small sample size needed
3. The sample testing procedure is simple and easy to operate
4. It can detect a wide variety of substances, including proteins, haptens, nucleic acids, amplification products, etc
5. Direct testing of liquid samples
6. No need for machines to interpret qualitative and semi-quantitative results
7. Qualified meet point-of-care testing
8. Long product validity period, no need for low temperature storage, reduce transportation and storage costs

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Cardiac Disease

Troponin I (cTnI)

Creatine kinase isoenzyme (CKMB)

Troponin I/myelin peroxidase (cTnI/MPO)

Troponin T (cTnT)

Cardiac Myosin-Binding Protein C (cMyBP-C)

Troponin I/N-terminal brain natriuretic peptide precursor /D-dimer (cTnI/NT-proBNP/DD)

Hypersensitivity troponin I (hs-cTnI)

Myelin peroxidase (MPO)

Growth Stimulation expressed gene 2 (ST2)

Hypersensitivity troponin T (hs-cTnT)

Troponin I/creatine kinase isoenzyme (cTnI/CKMB)

Soluble growth stimulates expression of gene 2 protein/N-terminal brain natriuretic peptide precursors (ST2/NT-proBNP)


Complement factor D (CFD)

Immunoglobulin E (IgE)

Antistreptolysin O IgG antibody (ASO Ab)

Complement factor H (CFH)

Proalbumin/C-reactive protein (PA/CRP)


Antistreptolytic O (ASO)

Prostate small leakage proteins (PSEP)


Pregnancy & Fertility

Estradiol (E2)

Anti-endometrial IgA (EmAb-IgA)

Pregnancy-associated plasma protein A (PAPP-A)

Follicle-stimulating hormone (FSH)

Anti-endometrial IgG (EmAb-IgG)

Fetal fibronectin (FFN)

Prolactin (PRL)

Anti-endometrial IgM (EmAb-IgM)

Placental growth factor (PLGF)

Testosterone (T)

Anti-human chorionic gonadotropin antibody (hCGAb)

Placental growth factor / soluble FMS like tyrosine kinase-1 (PLGF/sFlt-1)

Luteinizing hormone (LH)

Anti-zona pellucida antibody (AZPAb)

Inhibin B (INH-B)

Acrosomal protein SP-10 (ACRV1)

Anti cardiolipin / Anti-ovary / Anti-endometrial / Anti-sperm IgG

Free β- human chorionic gonadotropin (F-β-hCG)

Antisperm antibody IgA (AsAb-IgA)

Anti-trophoblast antibody (ATAb)

Alpha-fetoprotein (AFP)

Antisperm antibody IgG (AsAb-IgG)

Prolactin (PRL)

Human chorionic gonadotropins (hCG)

Antisperm antibody IgM (AsAb-IgM)

Ovulation hormones (Luteinizing hormone/Estrone–3-glucuronide) (LH/E3G)

β- Human chorionic gonadotropin core fragment / human chorionic gonadotropin (β-hCG/hCG)

Anti-ovarian antibody IgG (AOAb-IgG)

progesterone (P)

Alpha-fetoprotein/human chorionic gonadotropin free β  (AFP/β-hCG)

Anti-ovarian antibody IgM (AOAb-IgM)



Auto-immune Disease

Glial fibrous acidic protein (GFAP)

Antistreptolysin O/rheumatoid factor/C-reactive protein

Anticardiolipin antibody IgG (Cardiolipin IgG)

Anti-dSDNA antibody (Anti-dsDNA)

Anti-phospholipase A2 receptor antibody

Anticardiolipin antibody IgM (Cardiolipin IgM)

Anti-M2 mitochondrial antibody

Antiphospholipase A2 receptor antibody IgG

Anti-neutrophil cytoplasmic antibody (ANCA)

Anti-RA33 antibody

Anti-dSDNA antibody (Anti-dsDNA)

Anti-neutrophil cytoplasmic antibody IgG (ANCA IgG)

Antinuclear antibody (ANA)

Anti-dSDNA antibody IgG (Anti-dsDNA IgG)

Anti-tissue transglutaminase antibody IgA (Anti-tTG-IgA(ATA IgA))

Anti-cyclic citrulline peptide antibody (Anti-CCP)

Anti-mitochondrial antibody IgA/G/M (AMA-IgA/IgG/IgM)

Rheumatoid factor IgG (RF IgG)

Anti-cyclic citrulline peptide antibody / rheumatoid factor (Anti-CCP/RF)

Anti-mitochondrial antibody M2 type (AMA-M2)

Rheumatoid factor IgM (RF IgM)

Anti-cyclic citrulline polypeptide antibody IgG (Anti-CCP-IgG)

Anticardiolipin antibody (Anti-Cardiolipin)

Immunoglobulin G4 (IgG4)

Anti-keratin antibody

Anticardiolipin antibody IgA (Cardiolipin IgA)

Autoantibodies in vasculitis (ANCA/GPA/MPA)

Anti-soluble antigen



Therapeutic Drugs




Phenytoin (PHY)


Mycophenolic acid

Valproic acid (VPA)



theophylline (THE)

methotrexate (MTX)


digoxin (DIGN)

Carbamazepine (CAR)





Drug Abuse




Tetrahydrocannabinolic acid (THCA)



amphetamine (AMPH)

3,4  -Methylenedioxymethamphetamine (MDMA)


Benzodiazepines (BENZ)

6-Monoacetylmorphine (6-MAM)


Buprenorphine (BI Buprenorphine)



Infectious Disease

Group A streptococcus antigen

Mycoplasma pneumoniae IgM/IgG (MP IgM/IgG)

Measles virus IgM

Group B streptococcusantigens

Mycoplasma pneumoniae IgM (MP IgM)

Talaromyces marneffei antigen

Botulinum toxin type A (BtA)

Mycoplasma pneumoniae antigen (MP antigen)

Norovirus antigen (NV antigen)

Staphylococcal enterotoxin B (Enterotoxin type B) (SEB)

Parainfluenza virus IgG (PIV IgG)

Tetanus toxin IgG

Epstein-Barr Virus capsid antigen IgA (EBVCA-IgA)

Parainfluenza virus IgM (PIV IgM)

Mumps virus IgM

Epstein-Barr Virus capsid antigen IgM (EBVCA-IgM)

H7N9 antigen (H7N9)

Chlamydia trachomatis antigen (CT antigen)

Epstein-Barr Virus-Early Antigen IgG (EB-EA IgG)

Hantavirus IgM/IgG

Chlamydia trachomatis IgG (CT IgG)

Vibrio cholerae O139

Hantavirus IgM

Chlamydia trachomatis IgM (CT IgM)

Vibrio cholerae O1

Hantavirus IgG

Hemorrhagic fever with renal syndrome IgM (HFRS IgM)

Echo virus IgM (ECHOV IgM)

Respiratory syncytial virus antigen (RSV antigen)

Legionella pneumophila IgG (Lp IgG)

Hepatitis C virus IgG antibody (Anti-HCV IgG)

Hepatitis A virus IgG (HAV IgG)

Varicella-zoster virus IgM (VZV IgM)

Hepatitis C virus nucleic acid (HCV RNA)

Influenza A virus/influenza B virus/respiratory adenovirus/respiratory syncytial virus antigen

Parvovirus B19 IgG (B19 IgG)

Brucella antibody

Ureaplasma urealyticum IgG (UU IgG)

Parvovirus B19 IgM (B19 IgM)

Brucella antigen

Ureaplasma urealyticum IgM (UU IgM)

Hepatitis B virus e antibody (HBeAb)

Enterovirus 71 nucleic acid (EV71 RNA)

Coxsackie B virus IgG (CVB IgG)

Hepatitis B virus e antigen (HBeAg)

Dengue virus IgG/IgM (DENV IgG/IgM)

Coxsackie B virus IgM (CVB IgM)

Hepatitis B virus surface antibody (HBsAb)

Dengue virus NS1 antigen (DENV NS1)

Coxsackievirus A16 IgM (CA16 IgM)

Hepatitis B virus surface antigen/surface antibody/e antigen/e antibody/core antibody

Dengue virus NS1 antigen/IgG antibody/IgM antibody (DENV NSA1/IgG/IgM)

Rabies virus IgG (RV IgG)

Hepatitis B virus nucleic acid

Severe Fever with Thrombocytopenia Syndrome IgM/IgG (SFTS IgG/IgM)

Neisseria gonorrhoeae antigen (NG antigen)

Hepatitis B virus core antibody (HBcAb)

Streptococcal pneumoniae antigen (SP)

Neisseria gonorrhoeae / Chlamydia trachomatis antigen (NG/CA antigen)

Hepatitis B virus core antibody IgM (HBcAb IgM)

Chlamydia pneumoniae IgG (Cpn-IgG)

Influenza A virus IgM

Hepatitis B virus pre-S1 antigen (HBV-PreS1-Ag)

Chlamydia pneumoniae IgM (Cpn-IgM)

Influenza A virus IgM/Influenza B virus IgM/Parainfluenza virus IgM

Hepatitis B virus pre-S2 antigen (HBV-PreS2-Ag)

Chlamydia pneumoniae IgM / Mycoplasma pneumoniae IgM (Cpn-IgM/MP IgM)

Measles virus IgG

Hepatitis E virus IgG (HEV IgG)

Mycoplasma pneumoniae IgG (MP IgG)



New Born

Herpes simplex virus Type I IgM (HSV-1 IgM)

Rubella virus IgM (RV IgM)

Toxoplasma IgM/IgG,  Cytomegalo virus IgM/IgG, Rubella virus IgG (TORCH)

Herpes simplex virus Type II IgG (HSV-2 IgG)

Toxoplasma, Rubella virus, Cytomegalo virus, Herpes virus type I/type II IgG antibody (TORCH)

Cytomegalovirus IgG

Herpes simplex virus Type II IgM (HSV-2 IgM)

Toxoplasma, Rubella virus, Cytomegalo virus, Herpes virus type I/type II IgM antibody (TORCH)

Cytomegalovirus IgM

Herpes simplex virus Type II IgM/IgG (HSV-2 IgM/IgG)

Toxoplasma, Rubella virus, Cytomegalo virus, Herpes virus type II IgG (TORCH)

Herpes simplex virus type II IgM

Southeast Asia-deletion α-thalassemia Zeta-globin chain

Toxoplasma, Rubella virus, Cytomegalo virus, Herpes virus type II IgM (TORCH)

Measles virus IgG / Rubella virus IgG / Mumps virus IgG (MMR)

Rubella virus IgG (RV IgG)

Insulin-like growth factor binds to protein-1 (IGFBP-1)

IGF-binding protein-1/fetal fibronectin (IGFBP-1/FFN)

Free prostate-specific antigen (Free PSA)




Lymphotoxin α (LT-α)

Fecal occult blood (FOB)

Vascular endothelial growth factor (VEGF)

Carcinoembryonic antigen (CEA)

Chitosan 3-like protein 1

Golgi protein 73 (GP73)

Alpha-fetoprotein (AFP)

Prostate-specific antigen (PSA)

Carbohydrate antigen 15-3/Cancer antigen 15-3 (CA 15-3)

 Gynecological Disease

Candida albicans

Candida albicans antigen

Candida albicans/Trichomonas vaginalis/Gardnerella vaginalis antigen

Gardnerella vaginalis

Trichomonas vaginalis antigen

Candida albicans/vaginal trichomoniasis antigen

Liver Function

Type III. Procollagen

Collagen IV (Col4)

Cholyglycine (CG)

Procollagen 3 N-terminal peptide (PIIINP)


hyaluronic acid (HA)

Renal Function

β2-microglobulin (B2M(BMG))

Kidney injury molecule 1 (KIM-1)

Microalbumin (MAL)

Cystatin C (CysC)

Retinol-binding protein (RBP)

Microalbumin/creatinine (MAL/CRE)

Thyroid Disease

Thyroxine/Thyroid-Stimulating Hormone (TSH)

Thyroxine (T4)

Triiodothyronine (T3)

Thyroglobulin (TG)

Free thyroxine (Free T4)

Free triiodothyronine (Free T3)

Clinical Microbiology


Staphylococcus aureus

Bacillus anthracis antigen

Salmonella antigen

Gram-positive aerobic bacteria



Thrombin-activatable fibrinolysis inhibitor (TAFI)

Fibrin/Fibrinogen Degradation Products (FDP)


Vitamin B12 (VB12)

haemoglobin (Hb)



Anti-insulin antibody (AIAb)

Blood sugar

C-peptide / Insulin

Hemoglobin A1c (HbA1c)

Insulin (INS)


Dust mite allergen

Food-specific IgG (fIgG)

Digestive Disease

Lactoferrin (LF)






Adrenocorticotropic hormone (ACTH)




Bone Metabolism

Total Vitamin D

β-Crosslaps (β-CTX)

Procollagen type I N-propeptide (PINP)

Pancreatic Disease

Pancreatic elastase 1 (FE-1)


Alzheimer's disease

Amyloid-β (Aβ)

AD-associated neuronal thread protein (AD7c-NTP)

Blood Lipid

Total cholesterol (T-CHO)

Cardiovascular Disease

Lipoprotein(a)  (Lp (a))

Brain Injury

S100-β protein



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